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Effects of diets containing lycopene, tomato, and/or broccoli upon tumor growth and biomarkers in the Dunning R3327‐H prostate adenocarcinoma model
Author(s) -
CaneneAdams Kirstie,
Clinton Steven K.,
Wang Shihua,
Erdman John W.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a150-b
Subject(s) - lycopene , prostate cancer , carcinogenesis , endocrinology , medicine , adenocarcinoma , prostate , androgen , biology , chemistry , cancer , carotenoid , food science , hormone
The androgen‐dependent Dunning R3327‐H transplantable rat prostate adenocarcinoma model, in male Copenhagen rats, was utilized to examine dietary interventions on tumor growth and biomarkers. Diets were initiated 1 mo before tumor inoculation (20 mg of fresh tumor in Matrigel) and continued for ~18 wks. Rats showed similar food efficiency. Diets containing 10% broccoli ( P <0.01), 10% of tomato and 10% broccoli ( P <0.001), or following castration ( P <0.001), had significantly reduced tumor weights compared to control tumors. A greater than 40% decrease in tumor weights was observed in the rats fed 10% broccoli, whereas a 52% decrease was seen in the 10% broccoli with 10% tomato combination group, and castration resulted in a 62% decrease. Those fed 10% tomato or the combination of 5% tomato and broccoli powders showed a trend for reduction in tumor weights, with an almost 30% decline in growth compared to the control (for both, P <0.06). The high lycopene diet (0.25 g of 5% lycopene beadlets/kg diet) did not significantly reduce tumor weight ( P = 0.24). No physiologically relevant differences were seen in serum testosterone or dihydroxytestosterone concentrations. Blood and tissue samples were procured for assessment of biomarkers relevant to tumorigenesis using immunohistochemistry, gene expression via RT‐PCR, and P450 activity assays. (Supported by AICR #01B061/ IFAFS #00‐52101‐9695)