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Conjugated linoleic acid (CLA) might modify antioxidant system by different mechanism in the brain and liver of chronical ethanol‐treated rat
Author(s) -
Kim MinSeok,
Kim SeNa,
Park HyunSuh
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a146
Subject(s) - conjugated linoleic acid , cyp2e1 , chemistry , antioxidant , ethanol , liquid diet , glutathione peroxidase , glutathione , superoxide dismutase , biochemistry , medicine , endocrinology , linoleic acid , microsome , enzyme , biology , fatty acid
The study was designed to observe the effect of conjugated linoleic acid (CLA) on the antioxidative enzyme system in the brain and liver of chronically ethanol‐treated rats by Western blot analysis. Eighty male Sprague Dawley rats were divided into four groups; Control, ethanol(EtOH), CLA, ethanol and CLA (EtOH+CLA). All rats were fed Lieber‐DeCarli liquid diet for 4 weeks by pair‐feeding with those of EtOH group. The liquid diet was supplemented with CLA mixture at 0.8% by weight of the diet in the CLA‐treated groups and 50g ethanol (36%Kcal) was added in place of maltose dextrin for the ethanol‐treated groups. Ethanol significantly increased cytochrome P450 2E1 (CYP2E1), superoxide dismutase (CuZn‐SOD), glutathione peroxidase (GSH‐Px) and glutathione S‐transferase (GST) activities, but CLA supplementation significantly reduced CYP2E1 and CuZn‐SOD activities in brain of the EtOH+CLA group. Ethanol ingestion also significantly increased hepatic level of CYP2E1, whereas ethanol increased GST and reduced CuZn‐SOD activities, and had no effect on GSH‐Px level. CLA significantly increased CuZn‐SOD, GSH‐Px and GST activities in the EtOH+CLA group. Overall, CLA might modify antioxidant system by different mechanism to scavenge free radicals in the brain and liver. Supported by the Kyung Hee University Research Fund in 2005 (KHU‐20050419)