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HPLC‐ECD Bioanalysis of Tocopherol and CEHC Variants in Plasma of Patients with Diabetic Hypertension
Author(s) -
Williamson Kelly S.,
Gosmanova Albina K.,
Lyons Timothy J.,
Hensley Kenneth
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a144-d
Subject(s) - vitamin e , high performance liquid chromatography , tocopherol , diabetes mellitus , medicine , bioanalysis , chemistry , antioxidant , type 1 diabetes , alpha tocopherol , type 2 diabetes , endocrinology , chromatography , biochemistry
Purpose To assess plasma alpha tocopherol (αT), gamma tocopherol (γ T), delta tocopherol (δ T), tocol, 5‐nitro‐gamma tocopherol (5‐NO 2 ‐γ T), and carboxyethylhydroxychroman (α/γ‐CEHC) metabolites in patients with Type I/II diabetes (T1D/T2D) vs. non‐diabetics (ND), with and without hypertension. Methods Plasma samples were extracted and analyzed for tocopherol and CEHC analytes by high performance liquid chromatography coupled with electrochemical array detection (HPLC‐ECD). Results To date, 85 plasma samples showed no statistical association between αT and diabetes. However, there is a trend toward positive association of plasma γ T and γ‐CEHC with diabetes by ANOVA (T2D vs. ND, p<0.10). The plasma ratio γ T/αT increased in both T1D and T2D with a statistically significant difference in T2D vs. ND by unpaired 2‐tailed t‐test (p<0.04). Mean 5‐NO 2 ‐γ T was virtually identical in ND and T1D but increased non‐significantly in T2D (mean ± SEM: 25.4 ± 9.99 nM for ND; 22.3 ± 10.2 nM for T1D; 49.1 ± 16.7 nM for T2D). Conclusion The preliminary data suggests an alteration of plasma tocopherol distributions among diabetic subjects with increased levels of γ T, γ T/αT, γ‐CEHC, and 5‐NO 2 ‐γ T. Further sample collection and analysis is warranted with special attention given to possible interactions of diabetics with hypertensive conditions. Research support: American Diabetes Association (705RA56), OCAST (HR05051), and NCRR (M01 RR‐14467, OUHSC GCRC).