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Studies of PPARδ expression in fish oil and 9‐cis retinoic acid induction
Author(s) -
Chien Yiwen,
Lui KenNi,
Liao FangHsuean,
Shieh MingJer
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a139
Subject(s) - retinoic acid , fish oil , fish <actinopterygii> , chemistry , peroxisome proliferator activated receptor , expression (computer science) , microbiology and biotechnology , biology , biochemistry , fishery , receptor , computer science , gene , programming language
Many studies have indicated that fish oils had positive effects on prevention cardiovascular disease, but detail mechanisms are still unclear. PPARs are ligand‐activated transcription factors belonging to the nuclear receptor superfamily. They have three isoforms: PPARα, γ, and β/δ. All trans retinoic acids have been shown to increase uncoupling proteins expression in brown adipose tissue. and increase fatty acids β‐oxidation.. Thus, the aim of our study is to examine PPARδ expression in fish oil with or without 9‐cis retinoic acid induction. Forty C57BL/6J male mice were divided into four groups and were fed with soybean oil, fish oil, soybean oil plus all‐trans retinoic acids, fish oil plus all‐trans retinoic acids. After four months, serum and liver triglycerides, cholesterol, free fatty acids were analyzed. Tissues PPARs expression and key enzymes activity of fatty acids metabolism were also measured. The results indicated that fish oil group compare to soybean oil group had significantly increasing in PPARα, β/δ expression, decreasing in body weight and white adipose tissues. Also they had a lower triglycerides, cholesterol, free fatty acids concentration both in liver and serum. The activity of acetyl‐CoA oxidase (ACO) was increased. Fish oil with all‐trans retinoic acids group had more effective in lowering blood lipids, body weight, and increasing ACO activity and PPARα, β/δ expression level. In conclusion that all‐trans retinoic acids plus fish oil can significantly increase in PPARα, β/δ expression and activate key enzyme activity for fatty acids metabolism. It may be can decrease blood lipids and prevent cardiovascular disease or obesity.