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Effects of TNF‐α as a simulated stressor on adipose and liver tissues from rats fed varying diets
Author(s) -
Brown Andrew W,
Bohan Michelle M,
Mehyar Nimer,
Trenkle Allen H,
Beitz Donald C
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a126-c
Subject(s) - lipogenesis , lipolysis , endocrinology , medicine , adipose tissue , incubation , biology , fatty liver , lipid metabolism , chemistry , biochemistry , disease
Investigating the effects of a simulated stress on the tissues involved in lipid metabolism in rats subjected to varying diets could provide insight into the etiology of liver damage. Twelve adult male Sprague Dawley and 12 adult male Zucker Fatty rats were assigned randomly to one of three diets: Control, American Heart Association (AHA), or Atkins. Diets were fed for five weeks. Terminal liver and adipose tissue samples were harvested and subjected to an incubation period of two hours to assay lipolysis or lipogenesis. The tissues were incubated with either an incubation buffer or an incubation buffer treated with TNF‐α. Our results indicate that the TNF‐α effect in the lipogenesis of the liver tissue differs significantly (P ≤ 0.05) among breeds fed the different diets. TNF‐α increased lipogenesis in the livers of the Sprague Dawley rats on all diets, whereas TNF‐α increased liver lipogenesis in Zucker Fatty rats when fed the AHA diet but decreased liver lipogenesis when fed the Control and Atkins diets. No significant TNF‐α effect was shown on lipolysis in adipose or liver tissues, or on lipogenesis of adipose tissue (P>0.05). Thus, TNF‐α may promote fatty liver development via increased de novo lipogenesis in Sprague Dawley rats. Supported by the Burroughs Endowment