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Molecular genetic analysis of fish species for a commercial hatchery
Author(s) -
Butner Trevor L.,
Eckdahl Todd T.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a102
Subject(s) - rapd , biology , restriction fragment length polymorphism , restriction enzyme , hatchery , common carp , population genetics , population , mitochondrial dna , dna profiling , amplified fragment length polymorphism , zoology , fishery , fish <actinopterygii> , genetics , genetic diversity , polymerase chain reaction , dna , gene , cyprinus , demography , sociology
This research was initiated to address questions about the genetic relationships between several species of fish raised at Osage Catfisheries in Missouri. Arkansas chub, Arkansas black carp, and China black carp were studied using random amplified polymorphic DNA (RAPD) fingerprinting while restriction fragment length polymorphism (RFLP) fingerprinting was used to analyze paddlefish. The RAPD fingerprinting results similar molecular genetic fingerprints between the two species of carp, while the chub species differed. The D‐loop region of the paddlefish mitochondrial genome was amplified and cut with four restriction enzymes. Patterns were compared with already known polymorphisms from three populations: Missouri River at South Dakota, Osage River, and Osage Catfisheries. All restriction patterns of the paddlefish samples matched that of the Osage Catfisheries population. The combined results validate the use of molecular genetics methods in an industry‐driven application. This research was supported by the Missouri Western URSI program