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Lindane but not deltamethrin blocks a component of GABA‐activated chloride channels
Author(s) -
Ogata Nobukuni,
Vogel Stephen M.,
Narahashi Toshio
Publication year - 1988
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2.13.2458984
Subject(s) - deltamethrin , chemistry , lindane , pyrethroid , sodium channel , gabaa receptor , chloride channel , biophysics , chloride , reversal potential , gaba receptor , voltage clamp , receptor , sodium , patch clamp , biochemistry , pharmacology , biology , membrane potential , pesticide , organic chemistry , agronomy
Effects of deltamethrin, a pyrethroid insecticide, and lindane, the gamma isomer of hexachlorocyclohexane, on the γ‐aminobutyric acid (GABA)‐activated ion channels were studied using cultured neurons isolated from the newborn rat dorsal root ganglia. The neuron was voltage clamped using the whole cell patch clamp technique. Two components of inward chloride currents were generated in response to bath application of GABA. One of the components was selectively blocked by 1 × 10 −5 M lindane in a stereospecific manner, but neither component was affected by 1 × 10 −5 M deltamethrin, which drastically prolonged the voltage‐activated sodium channel current. Thus, the action of lindane to stimulate the nervous system is partly because of an interaction with the GABA receptor‐channel complex. The target site of deltamethrin is not the GABA receptor‐channel complex but the sodium channel. The results suggest a multiplicity of the GABA a receptor‐chloride channel complex.— O gata , N.; V ogel , S. M.; N arahashi , T. Lindane but not deltamethrin blocks a component of GABA‐activated chloride channels. FASEB J. 2: 2895‐2900; 1988.