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Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors
Author(s) -
Gabrielson Edward W.,
Gerwin Brenda I.,
Harris Curtis C.,
Roberts Anita B.,
Sporn Michael B.,
Lechner John F.
Publication year - 1988
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2.11.3260881
Subject(s) - dna synthesis , platelet derived growth factor receptor , platelet derived growth factor , epidermal growth factor , growth factor , mesothelial cell , stimulation , microbiology and biotechnology , cell growth , transforming growth factor , tgf alpha , cell culture , cell division , biology , endocrinology , chemistry , medicine , dna , cell , biochemistry , pathology , receptor , genetics
Monolayer cultures of human mesothelial cells made quiescent by serum deprivation are induced to undergo one round of DNA synthesis by platelet‐derived growth factor (PDGF), epidermal growth factor (EGF), or transforming growth factor type β1 (TGF‐β1). This onetime stimulation is independent of other serum components. The kinetics for induction of DNA synthesis observed for PDGF, EGF, and TGF‐β1 are all similar to one another, with a peak of DNA synthesis occurring 24‐36 h after the addition of the growth factors. Repetitive rounds of DNA synthesis and cell division do not ensue after addition of PDGF, EGF, or TGF‐β1 alone or in combination; however, in media supplemented with chemically denatured serum, each of these factors is capable of sustaining continuous replication of mesothelial cells. Stimulation of growth by PDGF and TGF‐β1 is unusual for an epithelial cell type, and indicates that mesothelial cells have growth regulatory properties similar to connective tissue cells.— G abrielson , E. W.; G erwin , B. I.; H arris , C. C.; R oberts , A. B.; S porn , M. B.; L echner , j . F. Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors. FASEB J. 2: 2717‐2721; 1988.