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Fresh and globular amyloid β protein (1–42) induces rapid cellular degeneration: evidence for AβP channel‐mediated cellular toxicity
Author(s) -
Bhatia Rajinder,
Lin Hai,
Lal Ratneshwar
Publication year - 2000
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.14.9.1233
Subject(s) - calcium , voltage dependent calcium channel , extracellular , chemistry , calcium channel , microbiology and biotechnology , confocal microscopy , amyloid (mycology) , biophysics , biology , pathology , biochemistry , medicine , inorganic chemistry , organic chemistry
Amyloid β peptides (AβP) deposit as plaques in vascular and parenchymal areas of Alzheimer's disease (AD) tissues and Down's syndrome patients. Although neuronal toxicity is a feature of late stages of AD, vascular pathology appears to be a feature of all stages of AD. Globular and nonfibrillar AβPs are continuously released during normal cellular metabolism, form calcium‐permeable channels, and alter cellular calcium level. We used atomic force microscopy, laser confocal microscopy, and calcium imaging to examine the real‐time and acute effects of fresh and globular AβP 1–42 , AβP 1–40 , and AβP 25–35 on cultured endothelial cells. AβPs induced morphological changes that were observed within minutes after AβP treatment and led to eventual cellular degeneration. Cellular morphological changes were most sensitive to AβP 1–42 .AβP 1–42 ‐induced morphological changes were observed at nanomolar concentrations and were accompanied by an elevated cellular calcium level. Morphological changes were prevented by anti‐AβP antibody, AβP‐channel antagonist zinc, and the removal of extracellular calcium, but not by tachykinin neuropeptide, voltage‐sensitive calcium channel blocker cadmium, or antioxidants DTT and Trolox. Thus, nanomolar fresh and globular AβP 1–42 induces rapid cellular degeneration by elevating intracellular calcium, most likely via calcium‐permeable AβP channels and not by its interaction with membrane receptors or by activating oxidative pathways. Such rapid degeneration also suggests that the plaques, and especially fibrillar AβPs, may not have a direct causative role in AD pathogenic cascades.—Bhatia, R., Lin H., Lal, R. Fresh and globular amyloid β protein (1–42) induces rapid cellular degeneration: evidence for Aβ P channel‐mediated cellular toxicity FASEB J . 14, 1233–1243 (2000)