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Entamoeba histolytica disturbs the tight junction complex in human enteric T84 cell layers
Author(s) -
Leroy Ancy,
Lauwaet Tineke,
De Bruyne Georges,
Cornelissen Maria,
Mareel Marc
Publication year - 2000
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.14.9.1139
Subject(s) - paracellular transport , tight junction , occludin , entamoeba histolytica , claudin , immunoprecipitation , microbiology and biotechnology , cell junction , biology , chemistry , permeability (electromagnetism) , cell , immunology , biochemistry , membrane , antibody
Entamoeba (E.) histolytica trophozoites initiate amebiasis through invasion into the enteric mucosa. It was our aim to understand the molecular interactions between amebic trophozoites and en‐terocytes during the early steps of invasion. Tropho‐zoites of E. histolytica strain HM1:IMSS were seeded on the apical side of enteric T84 cell layers, which were established on filters in two‐compartment culture chambers. Cocultures were analyzed for para‐cellular permeability by measurement of transepithe‐lial electrical resistance (TER) and for the tight junction proteins ZO‐1, ZO‐2, occludin, and cingulin by immunocytochemistry and immunoprecipitation. On direct contact with the apical side of the enteric cells, trophozoites caused an increase in paracellular permeability as evidenced by a decrease of TER associated with an increase in [ 3 H]mannitol flux. Immunoprecipitation of cocultures revealed dephos‐phorylation of ZO‐2, loss of ZO‐1 from ZO‐2, and degradation of ZO‐1 but less so of ZO‐2 and none of occludin or E‐cadherin. In conclusion, trophozoite‐associated increase in paracellular permeability of enteric cell layers is ascribed to disturbance of the molecular organization of tight junction proteins.—Leroy, A., Lauwaet, T., De Bruyne, G., Cornelissen, M., Mareel, M. Entamoeba histolytica disturbs the tight junction complex in human enteric T84 cell layers. FASEB J . 14, 1139–1146 (2000)