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Antibodies to the estrogen receptor‐α modulate rapid prolactin release from rat pituitary tumor cells through plasma membrane estrogen receptors
Author(s) -
NORFLEET ANDREA M.,
CLARKE CHARLOTTE H.,
GAMETCHU BAHIRU,
WATSON CHERYL S.
Publication year - 2000
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.14.1.157
Subject(s) - estrogen receptor , estrogen , prolactin , receptor , endocrinology , medicine , estrogen receptor beta , estrogen receptor alpha , chemistry , antibody , biology , microbiology and biotechnology , hormone , biochemistry , immunology , cancer , breast cancer
Antibodies (Abs) raised against the estrogen receptor‐α (ERα) were used to investigate the role of ERα proteins located at the plasma membrane in mediating the rapid, estrogen‐stimulated secretion of prolactin (PRL) from rat pituitary GH 3 /B6/F10 cells. Exposure of the cells to 1 nM 17 β‐estradiol (E 2 ) significantly increased PRL release after 3 or 6 min. When ERα Abs that bind specifically to ERα but are too large to diffuse into cells were tested for activity at the cell membrane, Ab R4, targeted to an ERα hinge region sequence, increased PRL release in a time‐ and concentration‐dependent fashion. Ab H151, directed against a different hinge region epitope, decreased PRL release and blocked the stimulatory action of E 2 . Abs raised against the DNA binding domain (H226) or the carboxyl terminus (C542) were not biologically active. When each Ab was examined for recognition of ERα on the cell surface by immunocytochemistry, all except H151 generated immunostaining in aldehyde‐fixed cells. In live cells, however, Ab H151 but not Ab R4 blocked the membrane binding of fluorescently tagged E 2 ‐BSA. Overall, the data indicate that plasma membrane ERα proteins mediate estrogen‐stimulated PRL release from GH 3 /B6/F10 cells. These results may also convey information about conformationally sensitive areas of the membrane form of ERα involved in rapid, nongenomic responses to estrogens.—Norfleet, A. M., Clarke, C. H., Gametchu, B., Watson, C. S. Antibodies to the estrogen receptor‐α modulate rapid prolactin release from rat pituitary tumor cells through plasma membrane estrogen receptors. FASEB J. 14, 157–165 (2000)