Premium
Annexin I modulates cell functions by controlling intracellular calcium release
Author(s) -
FREY BRIGITTE M.,
REBER BERNHARD F. X.,
VISHWANATH BANNIKUPPE S.,
ESCHER GENEVIEVE,
FREY FELIX J.
Publication year - 1999
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.13.15.2235
Subject(s) - calcium in biology , transfection , calcium , extracellular , intracellular , annexin , microbiology and biotechnology , annexin a2 , chemistry , biology , cell , biochemistry , organic chemistry , gene
Annexin I is an intracellular protein in search of a function. Ex vivo it has calcium‐ and phospholipid‐binding properties. To evaluate its role in vivo , MCF‐7 cells were stably transfected with annexin I in sense or antisense orientations. In cells overexpressing annexin I, calcium release was abrogated on stimulation of purinergic or bradykinin receptors, whereas non‐transfected cells or cells with down‐regulated annexin I released calcium within seconds. Basal calcium and calcium stores were not affected. The impaired calcium release was paralleled by a down‐regulation of the activities of phospholipase C, group II phospholipase A2, and E‐cadherin with altered adhesion and enhanced tumor growth on soft agar. Significantly smaller tumors, with the histologically most differentiated cells, were observed in nude mice inoculated with cells trans‐fected with the antisense rather than with the sense plasmid. These observations indicate that annexin I modulates cell functions by controlling intracellular calcium release. Frey, B. M., Reber, B. F. X., Vishwanath, B. S., Escher, G., Frey, F. J. Annexin I modulates cell functions by controlling intracellular calcium release. FASEB J. 13, 2235–2245 (1999)