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Costimulatory action of glycoinositolphospholipids from Trypanosoma cruzi: increased interleukin 2 secretion and induction of nuclear translocation of the nuclear factor of activated T cells 1
Author(s) -
Bellio Maria,
Oliveira AnaCarolina S. C.,
Mermelstein Claudia S.,
Capella Marcia A. M.,
Viola João P. B.,
Levraud JeanPierre,
Dosreis George A.,
Previato José O.,
MendonçaPreviato Lucia
Publication year - 1999
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.13.12.1627
Subject(s) - trypanosoma cruzi , secretion , microbiology and biotechnology , biology , t cell , t lymphocyte , interleukin 2 , cd3 , lymphocyte , immune system , cd8 , biochemistry , immunology , parasite hosting , world wide web , computer science
The effects of the glycoinositolphospholipids (GIPLs) from Trypanosoma cruzi on T lymphocyte activation were investigated in a mouse T cell hybridoma (DO‐11.10). Purified GIPLs from T. cruzi strains Y and G markedly increased IL‐2 mRNA transcripts and IL‐2 secretion induced by mitogenic anti‐CD3 and anti‐Thy1 mAbs. This costimulatory function was also revealed by the induction of IL‐2 secretion after the simultaneous addition of the T. cruzi GIPLs and either the calcium ionophore A23187 or phorbol ester. The capacity of the GIPL molecule to induce an increase in cytoplasmic calcium levels was also demonstrated. After exposure of T cell hybridoma to GIPL, the nuclear transcription factor NFAT1 became partially dephosphory‐lated, and its nuclear localization was demonstrated both in the T cell hybridoma and in Balb/c CD3 + cells. These results demonstrate that T. cruzi GIPL molecules are capable of signaling to T cells and therefore could be valuable tools for the study of T cell activation, besides playing a potential role in subverting the T lymphocyte immune response during T. cruzi infection.—Bellio, M., Oliveira, A.‐C. S. C., Mermelstein, C. S., Capella, M. A. M., Viola, J. P. B., Levraud, J.‐P., Dosreis, G. A., Pre‐viato, J. O., Mendonça‐Previato, L. Costimulatory action of glycoinositolphospholipids from Trypano‐soma cruzi: increased interleukin 2 secretion and induction of nuclear translocation of the nuclear factor of activated T cells 1. FASEB J . 13, 1627–1636 (1999)