Premium
Crystal‐induced neutrophil activation VI. Involvement of FcγRIIIB (CD16) and CD11b in response to inflammatory microcrystals
Author(s) -
Barabé Frédéric,
Gilbert Caroline,
Liao Nathalie,
Bourgoin Sylvain G.,
Naccache Paul H.
Publication year - 1998
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.12.2.209
Subject(s) - syk , tyrosine phosphorylation , phosphorylation , microbiology and biotechnology , chemistry , signal transduction , cd16 , integrin alpha m , protein tyrosine phosphatase , tyrosine kinase , tyrosine , stimulation , immunology , biochemistry , biology , immune system , receptor , endocrinology , cd3 , cd8
The inflammatory reaction associated with the deposition of monosodium urate (MSU) crystals in synovial spaces is known to be due to interactions with polymorphonuclear neutrophils mediated by presently unidentified surface structures. In this study, we have observed that antibodies directed against CD16 (VIFcRIII) and CD11b (VIM12) selectively and potently inhibit the activation of neutrophils by MSU crystals. The responses affected include the stimulation of tyrosine phosphorylation, activation of the tyrosine kinase syk , tyrosine phosphorylation of the proto‐oncogene Cbl, mobilization of calcium, and stimulation of the activity of phospholipase D and of the production of superoxide anions. Tyrosine phosphorylation responses to MSU crystals develop during the Me 2 SO 4 ‐induced differentiation of HL‐60 cells in parallel with the surface expression of CD16. These data strongly support the hypothesis that inflammatory microcrystals interact opportunistically with CD16 initially, and that the signal transduction pathways activated thereby depend on CD11b. An examination of the relevance of the hypothesis that an uncontrolled activation of CD16/CD11b may play a role in inflammatory reactions associated with a dysregulation of neutrophil function (other than crystal arthropathies) appears warranted on the basis of the present results.—Barabé, F., Gilbert, C., Liao, N., Bourgoin, S. G., Naccache, P. H. Crystal‐induced neutrophil activation: involvement of FcγRIIIB (CD16) in response to inflammatory microcrystals. FASEB J. 12, 209–220 (1998)