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Glutathione peroxidase protects mice from viral‐induced myocarditis
Author(s) -
Beck M. A.,
Esworthy R. S.,
Ho Y.S.,
Chu F.F.
Publication year - 1998
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.12.12.1143
Subject(s) - gpx1 , selenoprotein , glutathione peroxidase , viral myocarditis , selenium , gpx3 , myocarditis , gpx4 , virology , virus , coxsackievirus , chemistry , biology , microbiology and biotechnology , glutathione , enzyme , biochemistry , medicine , enterovirus , organic chemistry
Glutathione peroxidase 1 (GPX‐1) is a selenium‐dependent enzyme with antioxidant properties. Previous investigations determined that mice deficient in selenium developed myocarditis when infected with a benign strain of coxsackievirus B3 (CVB3/0). To determine whether this effect was mediated by GPX‐1, mice with a disrupted Gpx1 gene ( Gpx1 –/– ) were infected with CVB3/0. Gpx1 –/– mice developed myocarditis after CVB3/0 infection, whereas infected wild‐type mice (Gpx1 +/+ ) were resistant. Sequencing of viruses recovered from Gpx1 –/– ‐infected mice demonstrated seven nucleotide changes in the viral genome, of which three occurred at the G residue, the most easily oxidized base. No changes were found in virus isolated from Gpx1 +/+ mice. These results demonstrate that GPX‐1 provides protection against viral‐induced damage in vivo due to mutations in the viral genome of a benign virus.—Beck, M. A., Esworthy, R. S., Ho, Y.‐S., Chu, F.‐F. Glutathione peroxidase protects mice from viral‐induced myocarditis. FASEB J. 12, 1143–1149 (1998)