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Decrease of heart protein kinase C and cyclin‐dependent kinase precedes death in perinatal asphyxia of the rat
Author(s) -
Lubec Barbara,
Marx Manfred,
HerreraMarschitz Mario,
Labudova Olga,
Hoeger Harald,
Gille Lars,
Nohl Hans,
Mosgoeller Wilhelm,
Lubec Gert
Publication year - 1997
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.11.6.9194529
Subject(s) - protein kinase c , protein kinase a , programmed cell death , chemistry , lipid peroxidation , kinase , catalase , acidosis , superoxide dismutase , biochemistry , medicine , oxidative stress , apoptosis
Acidosis, energy depletion, overstimulation by excitatory amino acids, and free radical‐mediated reactions are the major current concepts for the explanation of damage and death resulting from asphyxia. Impaired phosphorylation by protein kinase C (PKC) represents another mechanism incriminated for cell death. We used an unsophisticated perinatal asphyxia model to study heart protein kinases PKC and cyclin dependent kinase (CDK). Tissue pH, ATP, the antioxidant enzymes superoxide dismutase, catalase, and glutathion peroxidase, lipid peroxidation products, carbonyls, and aromatic hydroxyiation were also tested. Electron spin resonance was applied to demonstrate the possible presence of radical adducts. An ELISA method was used to determine cell death. PKC activity and mRNA decreased with the length of the asphyctic periods and were paralleled by CDK and pH, whereas cell death gradually increased. No evidence was found for the involvement of active oxygen species or a radical adduct, and no energy depletion was observed. We conclude that impaired protein phosphorylation and/or acidosis may play a role in the pathobiochemistry of death from perinatal asphyxia in the rat.—Lubec, B., Marx, M., Herrera‐Marschitz, M., Labudova, O., Hoeger, H., Gille, L., Nohl, H., Mosgoeller, W., Lubec, G. Decrease of heart protein kinase C and cyclin‐dependent kinase precedes death in perinatal asphyxia of the rat FASEB J. 11, 482–492 (1997)