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Aerobic glycolysis by proliferating cells: a protective strategy against reactive oxygen species 1
Author(s) -
Brand Karl A.,
Hermfisse Ulrich
Publication year - 1997
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.11.5.9141507
Subject(s) - glycolysis , reactive oxygen species , anaerobic glycolysis , oxidative phosphorylation , chemistry , mitochondrion , microbiology and biotechnology , cell growth , biochemistry , context (archaeology) , biology , metabolism , paleontology
Our laboratory has reported that glucose is essential for glycolytic enzyme induction and proliferation of mitogen‐activated rat thymocytes (41). Here we show that: 1) Resting thymocytes meet their ATP demand mainly by oxidative glucose breakdown (88%), whereas proliferating thymocytes produce 86% by glycolytic degradation of glucose to lactate and only 14% by oxidation to CO 2 and water. 2) In contrast to nonstimulated resting thymocytes, production of PMA primed reactive oxygen species (ROS) in the proliferating cells is nearly abolished. 3) Consistent with this finding, no ROS formation is observed in proliferating human pro‐myelocytic HL‐60 cells, whereas differentiated, nonproliferating HL‐60 cells exert a marked response upon priming with PMA. 4) The observed reduction of ROS formation by resting thymocytes incubated with pyruvate suggests a function of pyruvate as an H 2 O 2 scavenger. 5) The respiratory chain is a potential origin for ROS because inhibitors of the mitochondrial electron transport strongly reduce H 2 O 2 production by resting thymocytes. The results are discussed in the context of aerobic glycolysis by proliferating cells being a means to minimize oxidative stress during the phases of the cell cycle where maximally enhanced biosynthesis and cell division do occur.—Brand, K. A., Hermfisse, U. Aerobic glycolysis by proliferating cells: a protective strategy against reactive oxygen species. FASEB J. 11, 388‐395 (1997)