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CD10 plays a specific role in early thymic development
Author(s) -
Guérin Sandrine,
Mari Bernard,
Maulon Laurence,
Belhacène Nathalie,
Marguet Didier,
Auberger Patrick
Publication year - 1997
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.11.5.9141505
Subject(s) - cd44 , cd8 , thymocyte , in vivo , population , t cell , t lymphocyte , microbiology and biotechnology , biology , in vitro , chemistry , medicine , endocrinology , immunology , biochemistry , antigen , immune system , genetics , environmental health
Development of T lymphocyte is a complex process that depends on both thymocyte‐stromal cell interactions and the production of soluble factors such as cytokines, peptides, and hormones. In many tissues, the concentration of active biological peptides is regulated locally by a specialized family of enzymes: the ectopeptidases. We show here that treatment of fetal thymic organ cultures (FTOC) with the specific CD 10 (endopeptidase 24.11) inhibitors SCH 32615: (N‐[L‐(1‐carboxy‐2‐phenyl)ethyl]‐L‐phenylalanyl‐β‐alanine), RB25: (N‐(3‐[(hydroaxyamino)carbonyl]‐2‐benzylidene‐1‐oxopropyl]‐N‐glycine), and thymopentin (TP5) results in the inhibition of thymocyte differentiation. Each agent induces a significant decrease in the number of double positive (CD4 + CD8 + ) cells in favor of the TN (TcRαβ − CD4 − CD8 − ) population. RB25 also blocks T lymphocyte differentiation in FTOC when preinjected into pregnant mice. Finally, RB25 and TP5 were also shown to reduce the number of CD44 + CD25 − and CD44 − CD25 − thymocytes both in vitro and after preinjection in vivo in day 2 FTOC. Thus, agents that affect endopeptidase 24.11 activity impair T cell development both in vitro and in vivo. Our results show that the CD 10 molecule plays a specific role in promoting early T cell development.—Guérin, S., Mari, B., Maulon, L, Belhacène, N., Marguet, D., Auberger, P. CD10 plays a specific role in early thymic development. FASEB J. 376‐381 (1997)