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Stem cell factor stimulates chicken osteoclast activity in vitro
Author(s) -
Hof R. J. Van 'T,
Von Lindern M.,
Nijweide P. J.,
Beug H.
Publication year - 1997
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.11.4.9068618
Subject(s) - osteoclast , stem cell factor , chemistry , haematopoiesis , stem cell , microbiology and biotechnology , cell culture , bone marrow , in vitro , biology , immunology , biochemistry , genetics
Stem cell factor (SCF) is a polypeptide growth factor active on multiple cell types, mainly of hematopoietic origin. We studied the effects of avian SCF on the differentiation of chicken osteoclasts from their putative progenitors as well as on the bone‐resorbing activity of terminally differentiated osteoclasts. Osteoclast formation was analyzed in long‐term cocultures of osteoblasts and nonadherent, osteoclast‐depleted bone marrow cells. Osteoclast activity was studied in short‐term (48 h) cultures of bone marrow cell populations enriched for osteoclasts, on dentine slices. SCF strongly enhanced osteoclast differentiation. The IL‐6‐related chicken myelomonocytic growth factor (cMGF) had a similar effect, and the effects of SCF and cMGF were additive. SCF, but not cMGF, also stimulated the bone‐resorbing activity of existing osteoclasts. As osteoblasts have been found to regulate osteoclast activity and formation, chicken osteoblasts were tested for their ability to express and secrete SCF. Reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis showed that osteoblasts express SCF mRNA and that parathyroid hormone increases expression levels about fourfold. SCF did not accumulate in the culture medium, but remained cell (osteoblasts) surface associated.—van't Hof, R. J., von Lindern, M., Nijweide, P. J., Beug, H. Stem cell factor stimulates chicken osteoclast activity in vitro. FASEB J. 11, 287‐293 (1997)