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Interferon‐inducible protein‐10 and lymphotactin induce the chemotaxis and mobilization of intracellular calcium in natural killer cells through pertussis toxin‐sensitive and ‐insensitive heterotrimeric G‐proteins
Author(s) -
Maghazachi Azzam A.,
Skålhegg Bjørn S.,
Rolstad Bent,
AlAoukaty Ala
Publication year - 1997
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.11.10.9271361
Subject(s) - pertussis toxin , chemotaxis , microbiology and biotechnology , heterotrimeric g protein , cxc chemokine receptors , chemokine , chemistry , g protein , chemokine receptor , cxcl14 , receptor , biology , signal transduction , biochemistry
We show here that interferon‐inducible protein‐10 (IP‐10), an ELR lacking CXC chemokine, and the C chemokine lymphotactin (Ltn) induce the chemotaxis and calcium mobilization in IL‐2‐activated NK (IANK) and CC chemokine‐activated NK (CHAK) cells. Cross‐desensitization experiments show that IP‐10 or Ltn use receptors not shared by other C, CC, or CXC chemokines. The chemotaxis induced by either IP‐10 or Ltn for both cell types is inhibited upon pretreatment of these cells with pertussis toxin (PT). Also, Ltn‐induced [Ca 2+ ] i in IANK but not in CHAK cells is inhibited upon pretreatment with PT, whereas IP‐10‐induced [Ca 2+ ] i in IANK and CHAK cells is inhibited upon pretreatment with this toxin. These results suggest important roles for PT‐sensitive and ‐insensitive G‐proteins in IP‐10‐induced and Ltn‐induced chemotaxis and calcium fluxes in activated NK cells. This was further implicated after streptolysin O permeabilization of CHAK and IANK cells and after introduction of inhibitory antibodies to the PT‐sensitive G i and G o or the PT‐insensitive G q . Our results suggest that IP‐10 and Ltn receptors are coupled to G i , G o , and G q in IANK cells and to G i and G q in CHAK cells, with a possible low coupling of IP‐10, but not of Ltn, receptors to G o in these cells. Together, these results show that IP‐10 and Ltn‐dependent chemotaxis and calcium mobilization may differentiate at the level of receptor coupling to the heterotrimeric G‐proteins.—Maghazachi, A. A., Skålhegg, B. S., Rolstad, B., Al‐Aoukaty, A. Interferon‐inducible protein‐10 and lymphotactin induce the chemotaxis and mobilization of intracellular calcium in natural killer cells through pertussis toxin‐sensitive and ‐insensitive heterotrimeric G‐proteins. FASEB J. 765–774 (1997)