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The making and breaking of symmetry in virus capsid assembly: glimpses of capsid biology from cryoelectron microscopy
Author(s) -
Steven Alasdair C.,
Trus Benes L.,
Booy Frank P.,
Cheng Naiqian,
Zlotnick Adam,
Caston Jose R.,
Conway James F.
Publication year - 1997
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.11.10.9271358
Subject(s) - capsid , biology , microscopy , capsomere , virus , virology , physics , optics
Virus capsids constitute a diverse and versatile family of protein‐bound containers and compartments ranging in diameter from ~200 Å (mass~1 MDa) to >1500 Å (mass> 250 MDa). Cryoelectron microscopy of capsids, now attaining resolutions down to 10 Å, is disclosing novel structural motifs, assembly mechanisms, and the precise locations of major epitopes. Capsids are essentially symmetric structures, and icosahedral surface lattices have proved to be widespread. However, many capsid proteins exhibit a remarkable propensity for symmetry breaking, whereby chemically identical subunits in distinct lattice sites have markedly different structures and packing relationships. Temporal differences in the conformation of a given subunit are also manifested in the large‐scale conformational changes that accompany capsid maturation. Larger and more complex capsids, such as DNA bacteriophages and herpes simplex virus, are formed not by simple self‐assembly, but under the control of tightly regulated programs that may include the involvement of viral scaffolding proteins and cellular chaperonins, maturational proteolysis, and conformational changes on an epic scale. In addition to its significance for virology, capsid‐related research has implications for biology in general, relating to the still largely obscure assembly processes of macromolecular complexes that perform many important cellular functions.—Steven, A. C., Trus, B. L., Booy, F. P., Cheng, N., Zlotnick, A., Caston, J. R., Conway, J. F. The making and breaking of symmetry in virus capsid assembly: glimpses of capsid biology from cryoelectron microscopy. FASEB J. 11, 733–742 (1997)