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Defective expression of cellular retinol binding protein type 1 and retinoic acid receptors α2, β2, and ǵ2 in human breast cancer cells
Author(s) -
Jing Yongkui,
Zhang Jie,
Bleiweiss Ira J.,
Waxman Samuel,
Zelent Arthur,
MiraYLopez Rafael
Publication year - 1996
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.10.9.8801168
Subject(s) - retinoic acid , retinoid , carcinogenesis , biology , signal transduction , cancer research , cell culture , retinoid x receptor gamma , retinoic acid receptor , receptor , microbiology and biotechnology , gene , biochemistry , genetics
Because the retinoic acid (RA) signaling pathway regulates cell proliferation and differentiation, inactivation of genes integral to the pathway represents a potential mechanism of carcinogenesis. We have studied in human breast cancer cells (T47D, MCF‐7, ZR75‐1, MDA‐MB‐231, and BT20) the expression of a subset of retinoid signaling genes that are themselves transcriptionally up‐regulated by RA, the cellular retinol binding protein type I (CRBPI) and the RA receptors (RARs) (α2, β2, and ǵ2. We find that constitutive expression of these genes is low or undetectable, and that expression levels are seldom responsive to 24 h treatment with 1 μM all‐trans or 9‐cis RA (Northern blot analysis). This is in contrast to breast fibroblasts, which show RA‐de‐ pendent expression of all four genes under the same conditions. Moreover, normal human breast epi‐thelial cells express CRBPI and RARβ2 at the mRNA level, suggesting that loss of expression of these genes is tied to malignant transformation. RARβ2, but not CRBPI, was also expressed in RA‐treated MTSV1‐7 cells, an immortalized but nontumorigenic luminal epithelial cell line. Lack of CRBPI and RARβ2 expression in cancer cells was not due to general impairment of RA signaling, as shown by RA activation of a RARE3‐tk‐CAT reporter in a subclone of MDA‐MB‐231 cells that did not express either CRBPI or RARβ2. These results suggest that at least two independent defects in the expression of proteins that function in retinoid signaling may be involved in breast carcinogenesis.—Jing, Y., Zhang, J., Blei‐ weiss, I. J., Waxman, S., Zelent, A., and Mira‐y‐ Lopez, R. Defective expression of cellular retinol binding protein type I and retinoic acid receptors a2, β2, and ǵ2 in human breast cancer cells. FASEB J. 10,1064‐1070 (1996)