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The stimulation of Na,K,Cl cotransport and of system A for neutral amino acid transport is a mechanism for cell volume increase during the cell cycle
Author(s) -
Bussolati Ovidio,
Uggeri Jacopo,
Belletti Silvana,
Dall'Asta Valeria,
Gazzola Gian C.
Publication year - 1996
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.10.8.8666170
Subject(s) - osmolyte , cotransporter , bumetanide , intracellular , chemistry , amino acid , biophysics , biochemistry , stimulation , cell cycle , cell , microbiology and biotechnology , sodium , biology , endocrinology , organic chemistry
It has been known for several years that the triggering of cell proliferation is associated with an increase of the activity of Na,K,Cl cotransport and of transport system A for neutral amino acids. These systems are also enhanced during the volume recovery of hypertonically shrunk cells. We demonstrate here that during the cell cycle of NIH3T3 cells, an increase in cell volume is associated with an enhanced cell content of potassium and amino acids. Bumetanide delays cell cycle progression and hampers volume increase. The non‐ metabolizable analog 2‐methylamino‐isobutyric acid, a specific substrate of system A, can partially substitute natural amino acids accumulated during the cell cycle as intracellular osmolytes. It is therefore proposed that the stimulation of Na,K,Cl cotransport and of system A, observed in proliferating cells, causes an expansion of cell volume through an enhanced intracellular accumulation of both in‐organic and organic osmolytes and the concurrent, osmotically obliged uptake of water.—Bussolati, O., Uggeri, J., Belletti, S., Dall'Asta, V., Gazzola, G. C. The stimulation of Na,K,Cl cotransport and of system A for neutral amino acid transport is a mechanism for cell volume increase during the cell cycle. FASEB J . 10, 920‐926 (1996)