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Mechanical load induces sarcoplasmic wounding and FGF release in differentiated human skeletal muscle cultures
Author(s) -
Clarke Mark S. F.,
Feerack Daniel L.
Publication year - 1996
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.10.4.8647349
Subject(s) - skeletal muscle , microbiology and biotechnology , fibroblast growth factor , chemistry , anatomy , medicine , biology , biochemistry , receptor
The transduction mechanism (or mechanisms) responsible for converting a mechanical load into a skeletal muscle growth response are unclear. In this study we have used a mechanically active tissue culture model of differentiated human skeletal muscle cells to investigate the relationship between mechanical load, sarcolemma wounding, fibroblast growth factor release, and skeletal muscle cell growth. Using the Flexcell Strain Unit we demonstrate that as mechanical load increases, so too does the amount of sarcolemma wounding. A similar relationship was also observed between the level of mechanical load inflicted on the cells and the amount of bFGF (FGF2) released into the surrounding medium. In addition, we demonstrate that the muscle cell growth response induced by chronic mechanical loading in culture can be inhibited by the presence of an antibody capable of neutralizing the biological activity of FGF. This study provides direct evidence that mechanically induced, sarcolemma wound‐me‐ diated FGF release is an important autocrine mechanism for transducing the stimulus of mechanical load into a skeletal muscle growth response.—Clarke, M. S. F., Feeback, D. L. Mechanical load induces sarcoplasmic wounding and FGF release in differentiated human skeletal muscle cultures. FASEB J . 10, 502‐509 (1996)