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Interleukin‐1 receptors on rat brain endothelial cells: a role in neuroimmune interaction?
Author(s) -
Van Dam A.M.,
De Vries H. E.,
Kuipeb J.,
Zijlstra F. J.,
De Boer A.G.,
Tilders F. J. H.,
Berkenbosch F.
Publication year - 1996
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.10.2.8641570
Subject(s) - receptor , medicine , endocrinology , cytokine , prostacyclin , interleukin , biology , inflammation , tumor necrosis factor alpha , prostaglandin e2 , endothelial stem cell , microbiology and biotechnology , in vitro , biochemistry
Inflammation following an infection induces a range of nonspecific symptoms of sickness in animals and humane. The cytokine interleukin‐1 (IL‐1) mediates many of the brain‐mediated symptoms of sickness. Binding sites for IL‐1 have been found in mouse brain, but not in the brains of rats. This raises questions as to the involvement of these neuronally localized IL‐1 binding sites in the induction of sickness symptoms. Based on observations of IL‐1 receptor mRNA in close vicinity to the vasculature in the mouse and rat brain, we studied the possibility that endothelial cells in the rat brain exhibit IL‐1 receptors to transduce information to the brain. Ligand binding studies reveal that cultured endothelial cells of adult rat brain exhibit specific binding sites for rat IL‐ 1β. Polymerase chain reaction experiments demonstrated that mRNA of the type I but not that of the type II IL‐1 receptor is present in rat brain endothelial cells. Incubation of these endothelial cells with recombinant rat IL‐1β showed a dose‐dependent increase in interleukin‐6, prostaglandin E 2, and prostacyclin secretion. Intravenous administration of rat IL‐1β to adult rats enhanced prostaglandin E 2 immunoreactivity in en‐dothelial cells of the brain microvasculature. These results indicate that functional type I IL‐1 receptors are present on endothelial cells of adult rat brain. We postulate that circulating IL‐1 can be translated by brain endothelial cells into other signals such as interleukin‐6 or prostaglandins that have access to the brain and induce sickness symptoms.—Van Dam, A.‐M., de Vries, H. E., Kuiper, J., Zijlstra, F. J., de Boer, A. G., Tilders, F. J. H., Berkenbosch, F. Interleukui‐1 receptors on rat brain endothelial cells: a role in neuroimmune interaction? FASEB J . 10, 351‐356 (1996)