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Electric stimulation of protein and DNA synthesis in human fibroblasts
Author(s) -
Bourguig Gerard J.,
Bourguig Lilly Y. W.
Publication year - 1987
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.1.5.3678699
Subject(s) - stimulation , pulse (music) , dna synthesis , dna , electrode , fibroblast , wi 38 , chemistry , biomedical engineering , voltage , biophysics , microbiology and biotechnology , materials science , biology , biochemistry , medicine , endocrinology , in vitro , gene , physics , quantum mechanics , ploidy
Human fibroblast cell cultures were employed as a model system to rapidly examine several potentially important variables involved in the use of high‐voltage, pulsed galvanic stimulation (HVPGS) to increase the healing rate of soft tissue injuries. Fibroblasts were grown on Millipore filters and exposed to HVPGS of various voltages and pulse rates for 20 min in a rectangular, plastic chamber filled with growth medium. Filters with attached cells were placed either in the center of the chamber or close to the positive or negative electrode. Protein synthesis and DNA synthesis were monitored after stimulation using the radioactively labeled precursors, [ 3 H]proline and [ 3 H]thymidine, respectively. The major results obtained in this study are as follows: 1 ) the rates of both protein and DNA synthesis can be significantly increased by specific combinations of HVPGS voltage and pulse rate; 2 ) maximum stimulation of protein and DNA synthesis was obtained at 50 and 75 V, respectively, with a pulse rate of 100 pulses/s and the cells located near the negative electrode; and 3 ) exposure to HVPGS intensities greater than 250 V (at all pulse rates and locations within the chamber) is inhibitory for both protein and DNA synthesis. In view of the results obtained in preliminary clinical studies on the use of HVPGS for the treatment of dermal ulcers, it appears that similar voltages, pulse rates, and relative electrode location may be required for maximum acceleration of human skin wound healing.— B ourguignon , G. J.; B ourguignon , L. Y. W. Electric stimulation of protein and DNA synthesis in human fibroblasts. FASEB J. 1: 398‐402; 1987.