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Expression of rat mRNA coding for hormone‐stimulated adenylate cyclase in Xenopus oocytes 1
Author(s) -
Smith Angel Anne,
Brooker Toni,
Brooker Gary
Publication year - 1987
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.1.5.2824269
Subject(s) - xenopus , adenylate kinase , cyclase , messenger rna , hormone , chemistry , microbiology and biotechnology , endocrinology , medicine , biology , gene , biochemistry , enzyme
β‐Adrenergic agonist‐stimulated hyperpolarization, whole‐cell cAMP accumulation, and activity of isoproterenol‐stimulated membrane‐bound adenylate cyclase (EC 4.6.1.1) in Xenopus laevis ovarian oocytes are entirely dependent on the presence of nascent follicle cells. A method was developed to remove rapidly and completely all extra‐oocyte cell types to yield defolliculated oocytes that exhibited normal viability and resting membrane potentials yet lacked β‐adrenergic receptor (βAR)‐stimulated responses. Purified follicle membranes contained βAR‐stimulated adenylate cyclase activity, whereas oocyte cell membranes did not. Purified oocyte membrane preparations from X. laevis oocytes previously microinjected with C6‐2B rat astrocytoma mRNA, and subsequently defolliculated, exhibited novel βAR and forskolin‐stimulated adenylate cyclase activity. These experiments demonstrate that oocytes expressed rat C6‐2B mRNA coding for the β‐adrenergic receptor and the components necessary for forskolin‐stimulated adenylate cyclase activity.— S mith , A. A.; B rooker , T.; B rooker , G. Expression of rat mRNA coding for hormone‐stimulated adenylate cyclase in Xenopus oocytes. FASEB J. 1: 380‐387; 1987.