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Net glucuronidation in different rat strains: importance of microsomal β‐glucuronidase
Author(s) -
Dwivedi C.,
Downie A. A.,
Webb T. E.
Publication year - 1987
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.1.4.3115856
Subject(s) - glucuronidation , microsome , bilirubin , chemistry , microsoma , carcinogen , biochemistry , enzyme , glucuronidase , glucuronosyltransferase , beta glucuronidase , compartmentalization (fire protection) , albumin , endocrinology , biology , gene , gene expression
The net glucuronidation of bilirubin (BR) has been determined in inbred and outbred rat strains and their subpopulations with similar glucuronosyltransferase (EC 2.4.1.17) activity but with different levels of β‐glucuronidase (βG) (EC 3.2.1.31), or in which the level of βG activity was reduced with d ‐glucaro‐1,4‐lactone. These studies demonstrated that outbred rat strains consist of two subpopulations that differ approximately 1.5‐ to twofold in serum and liver βG activity. Evidence is presented indicating that owing to its compartmentalization the lysosomal βG, unlike the corresponding microsomal enzyme, is neither inhibited by glucarolactone nor accessible for hydrolysis of newly synthesized glucuronides. The ratio of glucuronidated to unconjugated BR 15 min after injection of albumin‐bound BR into the tail vein appears to correlate negatively with the liver microsomal βG activity. The results may be relevant to the relative risk to toxins, including carcinogens, and to their reduction by dietary intervention.—D wivedi , C.; D ownie , A. A.; W ebb , T. E. Net glucuronidation in different rat strains: importance of microsomal β‐glucuronidase. FASEB J. 1: 303‐307; 1987.