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Protein kinase C in the regulation of smooth muscle contraction
Author(s) -
Rasmussen Howard,
Takuwa Yoh,
Park Susanna
Publication year - 1987
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.1.3.3040504
Subject(s) - myosin light chain kinase , caldesmon , myosin , calmodulin , tropomyosin , actin , contraction (grammar) , microbiology and biotechnology , muscle contraction , biophysics , phosphorylation , chemistry , biology , biochemistry , anatomy , endocrinology , enzyme
The cellular and molecular mechanisms underlying smooth muscle contraction are reviewed in the light of recent studies of smooth muscle ultrastructure and of the role of polyphosphoinositide turnover and protein kinase C function in smooth muscle contraction. A new model of smooth muscle contraction is proposed that differs radically from accepted views, particularly the latch bridge hypothesis, in terms of both Ca 2+ messenger function and the molecular events underlying this process. A coordinate fibrillar domain model of contraction is proposed in which the initial and sustained phases of contraction are mediated by different cellular and molecular events. The initial phase of response is mediated by a rise in [Ca 2+ ] c and the resulting calmodulin‐dependent activation of both myosin light chain kinase and the dissociation of caldesmon from the actin‐caldesmon‐tropomyosin‐myosin fibrillar domain. These events lead to an interaction between actin and the phosphorylated light chains of myosin just as in previous models. However, this initial phase is followed by a sustained phase in which a rise in [Ca 2+ ] sm stimulates the plasma membrane‐associated, Ca 2+ ‐sensitive form of protein kinase C that results in the phosphorylation of both structural and regulatory components of the filamin‐actin‐desmin fibrillar domain. These events underlie the tonic phase of contraction.— R asmussen , H.; T akuwa , Y.; P ark , S. Protein kinase C in the regulation of smooth muscle contraction. FASEB J. 1: 177‐185; 1987.