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Aging effects of vitamin C on a human lens protein produced in vitro
Author(s) -
Russell Paul,
Garland Donita,
Zigler J. Samuel,
Meakin Susan O.,
Tsui LapChee,
Breitman Martin L.
Publication year - 1987
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.1.1.3301496
Subject(s) - in vitro , chemistry , vitamin , lens (geology) , biochemistry , biology , paleontology
Human lens γ‐crystallin obtained from the expression of a gene construct stably integrated into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ‐crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may be responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.— R ussell , P.; G arland , D; Z igler , J. S., J r .; M eakin , S. O.; T sui , L.‐C.; B reitman , M. L. Aging effects of vitamin C on a human lens protein produced in vitro. FASEB J. 1: 32‐35; 1987.