Open AccessA High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain
Author(s) -
Lipu Wang,
Deborah Michel,
Wentao Zhang,
Anas ElAneed,
Pierre R. Fobert,
Yuefeng Ruan,
Samia Berraies,
Richard D. Cuthbert,
H. Randy Kutcher
Publication year - 2022
Publication title -
phytofrontiers
Language(s) - English
Resource type - Journals
ISSN - 2690-5442
DOI - 10.1094/phytofr-03-22-0024-ta
Subject(s) - fusarium , mycotoxin , germplasm , contamination , microbiology and biotechnology , wheat grain , biology , chromatography , environmental science , agronomy , chemistry , horticulture , ecology
Fusarium head blight (FHB), caused by Fusarium spp., is a destructive disease of cereal grains. Apart from grain yield loss, a major quality concern is contamination with Fusarium-produced mycotoxins, specifically deoxynivalenol (DON). Mycotoxins accumulate in the grain making it unfit for consumption by humans and animals. Breeding cultivars with high disease resistance and low mycotoxin contamination is a priority for wheat breeders. However, DON measurement in breeding programs is expensive and time consuming due to the lack of efficient quantification methods. In this study, we established a simple fast chromatography (FC) - tandem mass spectrometry (MS/MS) method, which employed a one-step acetonitrile extraction protocol with a short guard column to reduce complexity, cost and analysis time. To ensure robustness and reproducibility, the method was validated according to the US Food and Drug Administration Guidance for Bioanalytical Method Validation. Furthermore, the method was applied for determination of DON in 102 wheat grain samples. Obtained results highly correlated with the conventional immunological method for all tested samples. With its ease of use, rapid sample analysis, high sensitivity and accuracy, the method could be integrated into current FHB breeding programs to increase breeding efficiency and accelerate screening progress to identify germplasm with increased resistance to DON accumulation.