Open AccessMitogen-Activated Protein Kinase MAPKKK7 from Plasmodiophora brassicae Regulates Low-Light-Dependent Nicotiana benthamiana Immunity
Author(s) -
Chaofan Jin,
Rong Long,
Jing Zheng,
Xingyan Fang,
Wenming Wang,
Jing Fan,
Shu Yuan,
Junbo Du,
Hui Yang
Publication year - 2021
Publication title -
phytopathology
Language(s) - English
Resource type - Journals
eISSN - 1943-7684
pISSN - 0031-949X
DOI - 10.1094/phyto-08-20-0323-r
Subject(s) - nicotiana benthamiana , biology , agroinfiltration , hypersensitive response , microbiology and biotechnology , mapk/erk pathway , protein kinase a , map kinase kinase kinase , mapk cascade , kinase , programmed cell death , botany , virology , genetics , virus , apoptosis
MAPKKK is the largest family of mitogen-activated protein kinase (MAPK) cascades and is known to play important roles in plant pathogen interaction by regulating fungal cell proliferation, growth, and pathogenicity. Thus far, only a few have been characterized because of the functional redundancy of MAPKKKs. In this study, it is interesting that Plasmodiophora brassicae (Pb)MAPKKK7 was clustered into the A3 subgroup of plant MAPKKKs by a phylogenetic analysis and also with the BCK1 and STE groups of fungal MAPKKKs. PbMAPKKK7 function in reactive oxygen species accumulation and cell death in Nicotiana benthamiana was characterized. Agroinfiltration with the PbMAPKKK7 mutated protein kinase domain relieved these changes. Interestingly, the induction of cell death was dependent on light intensity. Transcriptional profiling analysis demonstrated that PbMAPKKK7 was highly expressed during cortex infection stages, indicating its important role in P. brassicae infection. These functional analyses of PbMAPKKK7 build knowledge of new roles of the MAPK cascade pathway in N. benthamiana and P. brassicae interactions.