Open AccessGenetic Diversity of Xylella fastidiosa in Mexican Vineyards
Author(s) -
Andrés Aguilar-Granados,
Bárbara Hernández-Macías,
Guillermo Santiago-Martínez,
Roberto Ruíz-Medrano,
Luis Kameyama-Kawabe,
Jesús Hinojosa-Moya,
María del Carmen Montes-Horcasitas,
Beatriz XoconostleCázares
Publication year - 2021
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pdis-09-20-1900-re
Subject(s) - xylella fastidiosa , biology , multilocus sequence typing , subspecies , genetic diversity , housekeeping gene , genotyping , botany , genotype , genetics , zoology , bacteria , gene , population , gene expression , demography , sociology
Xylella fastidiosa is a xylem-inhabiting phytopathogenic bacterium that affects diverse agriculturally relevant crops. In Mexico, X. fastidiosa has been reported in the states of Baja California, Coahuila, and Querétaro. In order to determine the genetic diversity of this bacterium in Mexico, 408 grapevine samples were collected from the main producing states in México. For X. fastidiosa identification, real-time PCR and three-loci end-point PCR were employed. The genotyping of the subspecies was carried out using multilocus sequence typing and analysis, based on seven housekeeping genes: leuA, petC, malF, cysG, holC, nuoL, and gltT. The resulting sequences were compared with those present in extant databases. The presence of X. fastidiosa subsp. fastidiosa in the states of Baja California (sequence type 1), Coahuila (sequence type 1), and Querétaro was confirmed. The isolates from northern Mexico bear high similarity to grapevine isolates from the United States. However, the isolates from Querétaro showed significant differences with currently known sequences, showing that there is genetic variability among the X. fastidiosa subsp. fastidiosa populations from grapevines in northern and central Mexico.