Open AccessFirst Report of Citrus tristeza virus in the State Union of Serbia and Montenegro
Author(s) -
Tatjana Papic,
Cláudia Simone Silveira dos Santos,
Gustavo Nolasco
Publication year - 2005
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pd-89-0434b
Subject(s) - citrus tristeza virus , biology , chlorosis , agarose gel electrophoresis , polymerase chain reaction , primer (cosmetics) , orange (colour) , rutaceae , plant virus , single strand conformation polymorphism , horticulture , virology , gene , virus , botany , genetics , chemistry , organic chemistry
Citrus production in the State Union of Serbia and Montenegro has a strategic
importance to the agricultural sector. Approximately 400,000 trees are now
grown in the major citrus producing region, which is the Montenegrin Coastal
Region. Satsuma mandarins and lemons grafted on
Poncirus trifoliata are the most cultivated varieties. In December 2003, eight samples taken from the
coastal region close to the towns of Bar and Ulcinj were analyzed using enzyme-
linked immunosorbent assay (ELISA) with SP7 antibodies produced at
Universidade do Algarve, Portugal (3). Further analysis was done using
immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR)
targeting the entire coat protein (CP) gene (forward primer CTV1: 5(prime)-
ATGGACGACGAAACAAAGAA-3(prime) and reverse primer CTV10: 5
(prime)-ATCAACGTGTGTTGAATTTCC-3(prime)). Using both techniques,
seven of eight samples analyzed were found to be infected by
Citrus tristeza virus (CTV), including samples from five trees that exhibited chlorosis,
gummosis, and fruit deformation, and two trees that were symptomless