Open AccessNatural Genetic Diversity in the Potato Resistance GeneRBConfers Suppression Avoidance fromPhytophthoraEffector IPI-O4
Author(s) -
Hari S. Karki,
Sidrat Abdullah,
Yu Chen,
Dennis Halterman
Publication year - 2021
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi-11-20-0313-r
Subject(s) - phytophthora infestans , nicotiana benthamiana , effector , r gene , biology , gene , genetics , plant disease resistance , blight , hypersensitive response , botany , microbiology and biotechnology
RB is a potato gene that provides resistance to a broad spectrum of genotypes of the late blight pathogen Phytophthora infestans. RB belongs to the CC-NB-LRR (coiled-coil, nucleotide-binding, leucine-rich repeat) class of resistance (R) genes, a major component of the plant immune system. The RB protein detects the presence of class I and II IPI-O effectors from P. infestans to initiate a hypersensitive resistance response, but this activity is suppressed in the presence of the Class III effector IPI-O4. Using natural genetic variation of RB within potato wild relatives, we identified two amino acids in the CC domain that alter interactions needed for suppression of resistance by IPI-O4. We have found that separate modification of these amino acids in RB can diminish or expand the resistance capability of this protein against P. infestans in both Nicotiana benthamiana and potato. Our results demonstrate that increased knowledge of the molecular mechanisms that determine resistance activation and R protein suppression by effectors can be utilized to tailor-engineer genes with the potential to provide increased durability. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .