Open AccessLong-Read Genome Sequence Resource of Ascochyta versabilis Causing Leaf Spot Disease in Pseudostellaria heterophylla
Author(s) -
Yun-Bo Kuang,
Zhe Wang,
Abah Felix,
Hongli Hu,
Baohua Wang,
Zonghua Wang,
Huiping Zhang,
Zuyun Ye,
Jiandong Bao
Publication year - 2020
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi-05-20-0137-a
Subject(s) - biology , genome , ascochyta , whole genome sequencing , contig , gene , single molecule real time sequencing , sequence assembly , leaf spot , genetics , dna sequencing , computational biology , botany , blight , transcriptome , gene expression , dna sequencer
Ascochyta versabilis is the fungal pathogen that causes the severe leaf spot disease of Pseudostellaria heterophylla (Miq.) Pax, a vital Chinese herbal plant. Here, we deployed PacBio single-molecule real-time long-read sequencing technology to generate a near-complete genome assembly for the A. versabilis KC1 strain and obtained a total of 9.80 Gb raw reads. These reads were processed into a 41.05 Mb genome assembly containing 95 contigs with N50 of 1.70 Mb and a maximum length of 3.93 Mb. A total of 10,457 gene models, of which 1,004 encode putatively secreted proteins, were identified in the genome. This high-quality genome assembly and gene annotation resource will facilitate the institution of functional genetic studies aimed at providing a better insight into the infection mechanisms of A. versabilis to support the development of effective control strategies for leaf spot disease of P. heterophylla.