Open AccessThree Common Symbiotic ABC Subfamily B Transporters inMedicago truncatulaAre Regulated by a NIN-Independent Branch of the Symbiosis Signaling Pathway
Author(s) -
Sonali Roy,
Andrew Breakspear,
Donna Cousins,
Ivone TorresJerez,
Kirsty Jackson,
Anil Kumar,
Yangyang Su,
ChengWu Liu,
Nick Krom,
Michael K. Udvardi,
Ping Xu,
Jeremy D. Murray
Publication year - 2021
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi-02-21-0036-r
Subject(s) - medicago truncatula , rhizobia , biology , subfamily , symbiosis , atp binding cassette transporter , mutant , root hair , gene , nod factor , microbiology and biotechnology , genetics , transporter , bacteria
Several ATP-binding cassette (ABC) transporters involved in the arbuscular mycorrhizal symbiosis and nodulation have been identified. We describe three previously unreported ABC subfamily B transporters, named AMN1, AMN2, and AMN3 (ABCB for mycorrhization and nodulation), that are expressed early during infection by rhizobia and arbuscular mycorrhizal fungi. These ABCB transporters are strongly expressed in symbiotically infected tissues, including in root-hair cells with rhizobial infection threads and arbusculated cells. During nodulation, the expression of these genes is highly induced by rhizobia and purified Nod factors and is dependent on DMI3 but is not dependent on other known major regulators of infection, such as NIN, NSP1, or NSP2. During mycorrhization their expression is dependent on DMI3 and RAM1 but not on NSP1 and NSP2. Therefore, they may be commonly regulated through a distinct branch of the common symbiotic pathway. Mutants with exonic Tnt1-transposon insertions were isolated for all three genes. None of the single or double mutants showed any differences in colonization by either rhizobia or mycorrhizal fungi, but the triple amn1 amn2 amn3 mutant showed an increase in nodule number. Further studies are needed to identify potential substrates of these transporters and understand their roles in these beneficial symbioses. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .