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Determination of Molecular Weights of C Hordeins by Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI‐MS)
Author(s) -
Savage A. W. J.,
Saletti R.,
Foti S.,
Shewry P. R.,
Tatham A. S.
Publication year - 2002
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem.2002.79.6.768
Subject(s) - chemistry , mass spectrometry , matrix assisted laser desorption/ionization , molecular mass , chromatography , maldi imaging , matrix (chemical analysis) , desorption , biochemistry , adsorption , organic chemistry , enzyme
The molecular weights of nine C hordein proteins, including all of the major components identified by SDS‐PAGE have been determined by MALDI‐MS. Most were in the range 43,561 to 46,857, with a single component of M r 57,606. These are higher than the masses previously reported based on the sequences of cloned genes. This indicates that reliance on masses determined solely by gene sequencing is not valid, presumably because these may not encode the major expressed proteins.