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Fate of Dwarf Bunt Fungus Teliospores During Milling of Wheat into Flour
Author(s) -
Bechtel Donald B.,
Wilson Jeff D.,
Eustace W. D.,
Behnke Keith C.,
Whitaker Thomas,
Peterson Gary L.,
Sauer David B.
Publication year - 1999
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem.1999.76.2.270
Subject(s) - spore , bran , wheat flour , chemistry , sieve (category theory) , contamination , pellet , fungus , food science , biology , botany , mathematics , raw material , ecology , organic chemistry , combinatorics , zoology
Wheat contaminated with teliospores of Tilletia controversa Kühn (TCK) was mixed with uncontaminated wheat and processed through the Kansas State University pilot mill. Two 50‐bu lots of the contaminated mixture were cleaned, tempered, and milled. Approximately 500 samples of wheat, cleanings, and mill fractions were collected and examined for the presence of intact and broken TCK teliospores. Whole wheat samples (50 g) were washed, sieved through a 60‐μm nylon sieve, and pelleted by centrifugation. Contents of the pellet were examined microscopically for the presence of TCK spores. The procedure was modified as needed to accommodate cleanings and mill fractions. Levels of spore contamination in whole wheat samples decreased at each step during the handling process, and large numbers of spores were found in materials that were sieved or aspirated from the grain. Very few spores were found in bran, germ, and shorts; none were detected in red dog or straight‐grade flour. The results showed that a high percentage of spores can be removed from wheat by mechanical cleaning but that it is not feasible to remove all of them.