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Determination of Damaged Starch and Diastatic Activity in Wheat Flour Using a Flow‐Injection Analysis Biosensor Method
Author(s) -
Haginoya Ryuichi,
Sakai Kunio,
Komatsu Takashi,
Nagao Seiichi,
Yokoyama Kenji,
Takeuchi Toshifumi,
Matsukawa Ritsuko,
Karube Isao
Publication year - 1997
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem.1997.74.6.745
Subject(s) - biosensor , glucose oxidase , chemistry , starch , chromatography , detection limit , flow injection analysis , wheat flour , immobilized enzyme , reproducibility , biochemistry , enzyme , food science
The determination of damaged starch and diastatic activity in flour was studied using a flow‐injection analysis (FIA) biosensor system. The system consisted of an oxygen electrode and an immobilized enzyme column containing purified glucoamylase and glucose oxidase immobilized on activated aminopropyl glass beads. The biosensor system has an optimum pH between 6.5 and 7.5 and an optimum temperature of 35°C for glucose measurement. The response of the FIA biosensor was linear up to 1.000 g/L of glucose with a lower detection limit of 0.025 g/L. Each assay took about 20 min, and the system showed good reproducibility ( r = 0.998, n = 8). When applied to the measurement of damaged starch and diastatic activity in wheat flour, the results obtained agreed with those obtained using the conventional methods of measurement. This biosensor system is a rapid practical alternative for the measurement of damaged starch and diastatic activity in wheat flour.