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Detection of Irradiated Wheat Using the Electron Paramagnetic Resonance Spin Probe Technique
Author(s) -
Dadayli Dilek,
Sünnetçioğlu M. Maral,
Köksel Hamit,
Çelik Süeda
Publication year - 1997
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem.1997.74.4.375
Subject(s) - electron paramagnetic resonance , chemistry , potassium ferricyanide , irradiation , aqueous solution , analytical chemistry (journal) , ferricyanide , paramagnetism , pulsed epr , spin probe , spectral line , nuclear magnetic resonance , spin echo , inorganic chemistry , chromatography , magnetic resonance imaging , condensed matter physics , medicine , physics , astronomy , nuclear physics , radiology
In this study, the electron paramagnetic resonance (EPR) spin probe technique is applied as a detection method in the differentiation of irradiated and unirradiated wheat seeds. Two wheat cultivars, Kunduru and Bezostaya, were used. Aqueous solutions of 4‐hydroxy‐TEMPO (TANOL) spin probe prepared with a line‐broadening agent, potassium ferricyanide, were used in all experiments. The EPR spectra of the samples were recorded against time. A decrease in the signal intensity and a change in the shape of the intensity‐time curve (rehydration curve) were observed, depending on the applied level of irradiation. The ratio of the lipid and aqueous regions at the high field (m I = ‐1) line changes, depending on the dose of irradiation.