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Influence of Low Molecular Weight Glutenins on Viscoelastic Properties of Intact Wheat Kernels and Their Relation to Functional Properties of Wheat Dough
Author(s) -
Maucher Tanja,
Figueroa J. D. C.,
Reule W.,
Peña R. J.
Publication year - 2009
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem-86-4-0372
Subject(s) - glutenin , chemistry , gluten , food science , protein subunit , biochemistry , gene
The mechanical and viscoelastic properties of intact wheat kernels of 36 wheat cultivars differing in low molecular weight glutenin subunit (LMW‐GS) composition (loci Glu‐A3, Glu‐B3 , and Glu‐D3 ) were evaluated using load‐compression tests. Comparison among genotypic groups representing Glu‐3 allelic variants showed that groups representing the alleles Glu‐A3 b, c, and d; Glu‐B3 d, g, and h; and Glu‐D3 a, b, and d, had harder kernel texture, higher kernel elastic work and larger gluten strength‐related parameters than those possessing alleles Glu‐A3 e; Glu‐B3 f, i and j (translocation 1B/1R); and Glu‐D3 d. Modulus of elasticity (stress to strain ratio) showed low values (111.9–168.8 MPa) for allelic groups possessing poor elastic properties ( Glu‐A3 e; Glu‐B3 f, i, and j; and Glu‐D3 d), and high values (179.8–222.6 MPa) for allelic groups possessing high kernel elastic properties ( Glu‐A3 b c, and d; Glu‐B3 d, g, and h; and Glu‐D3 a, b and c). The highest values for gluten strength‐related parameters (SDS‐sedimentation, dough mixing time, and dough strength [ W ]) corresponded to allelic groups Glu‐A3 d; Glu‐B3 d and g; and Glu‐D3 d, while the lowest corresponded to Glu‐A3 e and Glu‐B3 j. No significant differences were observed among groups with regard to gluten extensibility parameters; however, the highest P/L value (least extensibility) corresponded to Glu‐B3 j, which indicates presence of 1B/1R translocation. Except for the Glu‐B3 j (translocation 1B/1R) allele, which presented more variation within samples, a general relationship between kernel viscoelastic properties and dough viscoelastic properties was observed; samples showing higher elastic work to plastic work ratio ( E/P ) tended to possess better gluten strength than cultivars with low E/P ratio.

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