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Isolation of an Antifungal Pathogenesis‐Related Protein from Naked Oat ( Avena nuda ) Seeds
Author(s) -
Shi Yawei,
Jian Liu,
Han Deping,
Ren Yemei
Publication year - 2015
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem-12-13-0251-r
Subject(s) - chemistry , size exclusion chromatography , chromatography , avena , acetone , ion chromatography , molecular mass , affinity chromatography , biochemistry , botany , enzyme , biology
A novel antifungal protein was isolated from naked oat ( Avena nuda ) seeds by acetone fractionation, (NH 4 ) 2 SO 4 precipitation, Q‐Sepharose ion‐exchange chromatography, chitin affinity chromatography, and Sephacryl S‐200 gel filtration chromatography. The antifungal protein exhibited a molecular mass of 23,760, as measured by gel filtration and SDS‐PAGE. Matrix‐assisted laser desorption ionization–time of flight MS analysis indicated that the protein was homologous with a permatin precursor from A. sativa . The protein from naked oats exhibited antifungal activity against Trametes sp. SQ01 (half‐maximal inhibitory concentration [IC 50 ] = 5.68μM), Trichoderma spp. (IC 50 = 0.83μM), and Chaetomium sp. R01.