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Modified Orcinol–Ferric Chloride Colorimetric Assay for Water‐Extractable Arabinoxylan in Wheat
Author(s) -
Hu Gongshe,
Ellberg Sherry,
Burton Charlotte,
Evans Chris,
Satterfield Kathy
Publication year - 2017
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem-09-16-0230-r
Subject(s) - chemistry , arabinoxylan , ferric , orcinol , chloride , turbidity , food science , chromatography , biochemistry , polysaccharide , organic chemistry , oceanography , geology
Arabinoxylans are an important quality component for which a simple and rapid analysis method useful for plant breeding needs to be developed. Bial's orcinol–ferric chloride colorimetric method was modified to allow simultaneous analysis of at least 24 samples in a single run and in small volumes to measure water‐extractable arabinoxylan (WEAX) in wheat meal. The modified parameters were reduction to 6N HCl, increased concentrations of orcinol and 10% ferric chloride, and a total volume of 500 µL. The sample turbidity was reduced by rapid cooling in an ice water bath followed by addition of 500 µL of 95% ethanol postincubation. The WEAX results from this protocol were compared with GC/MS values. The wheat varieties of Alta Blanca, Eltan, and Hatton were overestimated by 4.3, 1.5, and 0.9%, respectively, whereas the varieties of Eden, Darwin, Finley, and Boundary were underestimated by 14.4, 8.9, 11.3, and 3.6%, respectively. The modified procedure is useful to determine a range of WEAX concentrations in wheat grains.