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Response to the Views and Opinions of Maningat, Seib, and Bassi Regarding McCleary et al (2013)
Author(s) -
McCleary Barry V.
Publication year - 2013
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1094/cchem-06-13-0117-vo
Subject(s) - chemistry , amylase , starch , food science , dietary fiber , hydrolysis , biochemistry , enzyme
Phosphate cross‐linked starch, referred to as resistant starch 4 (RS 4 ) is hydrolyzed much more rapidly under physiological conditions (i.e., pH 6, 37°C, pancreatic α‐amylase) than under the conditions used in AOAC dietary fiber method 985.29/AACC International Approved Method 32‐45.01 (the Prosky method), in which samples are incubated with thermostable bacterial α‐amylase at 95–100°C. For this reason, the author concludes that the Prosky method overestimates the dietary fiber content of these materials. A more accurate estimate of dietary fiber content is obtained with AOAC Method 2009.01/AACCI Approved Method 32‐45.01.