Open AccessCharacterization and biological evaluation of a novel silver nanoparticle-loaded collagen-chitosan dressing
Author(s) -
Rongfu Li,
Zhaorong Xu,
Qiong Jiang,
Yunquan Zheng,
Zhaohong Chen,
Xiaodong Chen
Publication year - 2020
Publication title -
regenerative biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.166
H-Index - 25
ISSN - 2056-3426
DOI - 10.1093/rb/rbaa008
Subject(s) - chitosan , wound healing , biocompatibility , chemistry , transforming growth factor , growth factor , vascular endothelial growth factor , cytokine , basic fibroblast growth factor , epidermal growth factor , umbilical vein , platelet derived growth factor receptor , platelet derived growth factor , silver nanoparticle , biomedical engineering , pharmacology , microbiology and biotechnology , materials science , immunology , nanoparticle , nanotechnology , biochemistry , in vitro , cancer research , vegf receptors , medicine , biology , receptor , organic chemistry
Effective coverage and protection is a priority in wound treatment. Collagen and chitosan have been widely used for wound dressings due to their excellent biological activity and biocompatibility. Silver nanoparticles (AgNPs) have a powerful antibacterial effect. In this study, a macromolecular and small-molecular collagen mixed solution, a macromolecular and small-molecular chitosan mixed solution were prepared, and a silver nanoparticle-loaded collagen-chitosan dressing (AgNP-CCD) has been proposed. First, the effects of a collagen-chitosan mixed solution on the proliferation of human umbilical vein endothelial cells and the secretion of cytokines were evaluated. Then, the characteristics and antibacterial effects of the AgNP-CCD were tested, and the effects on wound healing and the influence of wound cytokine expression were investigated via a deep second-degree burn wound model. The results showed that at the proper proportion and concentration, the collagen-chitosan mixed solution effectively promoted cell proliferation and regulated the levels of growth factors (vascular endothelial growth factor [VEGF], epidermal growth factor [EGF], platelet-derived growth factor [PDGF], transforming growth factor [TGF-β1], basic fibroblastic growth factor [bFGF]) and inflammatory factors (TNF-α, IL-1β, IL-6, IL-8). Moreover, AgNP solutions at lower concentrations exerted limited inhibitory effects on cell proliferation and had no effect on cytokine secretion. The AgNP-CCD demonstrated satisfactory morphological and physical properties as well as efficient antibacterial activities. An in vivo evaluation indicated that AgNP-CCD could accelerate the healing process of deep second-degree burn wounds and played an important role in the regulation of growth and inflammatory factors, including VEGF, EGFL-7, TGF-β1, bFGF, TNF-α and IL-1β. This AgNP-CCD exerted excellent biological effects on wound healing promotion and cytokine expression regulation.