Open AccessDynamics of intracellular stress-induced tRNA trafficking
Author(s) -
Rabin Dhakal,
Chunyi Tong,
Seán T. Anderson,
Anna Kashina,
Barry S. Cooperman,
Haim H. Bau
Publication year - 2018
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gky1208
Subject(s) - biology , cytoplasm , transfer rna , chromosomal translocation , microbiology and biotechnology , ribosome , nocodazole , nuclear export signal , puromycin , translation (biology) , cell nucleus , intracellular , protein biosynthesis , biochemistry , nucleus , extracellular , rna , cell , messenger rna , cytoskeleton , gene
Stress is known to induce retrograde tRNA translocation from the cytoplasm to the nucleus but translocation kinetics and tRNA-spatial distribution have not been characterized previously. We microinject fluorescently-labeled tRNA into living cells and use confocal microscopy to image tRNA spatial distribution in single cells at various levels of starvation and to determine translocation rate constants. Retrograde tRNA translocation occurs reversibly, within minutes after nutrition depletion of the extracellular medium. Such nutritional starvation leads to down-regulation of tRNA nuclear import and nearly complete curtailment of its nuclear export. Nuclear tRNA accumulation is suppressed in cells treated with the translation inhibitor puromycin, but is enhanced in cells treated with the microtubule inhibitor nocodazole. tRNA in the cytoplasm exhibits distinct spatial distribution inconsistent with diffusion, implying that such distribution is actively maintained. We propose that tRNA biological complexes and/or cytoplasmic electric fields are the likely regulators of cytoplasmic tRNA spatial distribution.