Open AccessSuper-enhancer-associated MEIS1 promotes transcriptional dysregulation in Ewing sarcoma in co-operation with EWS-FLI1
Author(s) -
Litian Lin,
Moli Huang,
Xianping Shi,
Anand Mayakonda,
Kaishun Hu,
YanYi Jiang,
Xiangmin Guo,
Li Chen,
Brendan Pang,
Ngan Doan,
Jonathan Said,
JianJun Xie,
Sigal Gery,
ChongYu Xu,
Zhaoyu Lin,
Jinsong Li,
Benjamin P. Berman,
Yin Ding,
DeChen Lin,
H. Phillip Koeffler
Publication year - 2018
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gky1207
Subject(s) - enhancer , biology , fli1 , cancer research , transcription factor , sarcoma , transcriptional regulation , regulation of gene expression , oncogene , gene , genetics , medicine , pathology , cell cycle
As the second most common malignant bone tumor in children and adolescents, Ewing sarcoma is initiated and exacerbated by a chimeric oncoprotein, most commonly, EWS-FLI1. In this study, we apply epigenomic analysis to characterize the transcription dysregulation in this cancer, focusing on the investigation of super-enhancer and its associated transcriptional regulatory mechanisms. We demonstrate that super-enhancer-associated transcripts are significantly enriched in EWS-FLI1 target genes, contribute to the aberrant transcriptional network of the disease, and mediate the exceptional sensitivity of Ewing sarcoma to transcriptional inhibition. Through integrative analysis, we identify MEIS1 as a super-enhancer-driven oncogene, which co-operates with EWS-FLI1 in transcriptional regulation, and plays a key pro-survival role in Ewing sarcoma. Moreover, APCDD1, another super-enhancer-associated gene, acting as a downstream target of both MEIS1 and EWS-FLI1, is also characterized as a novel tumor-promoting factor in this malignancy. These data delineate super-enhancer-mediated transcriptional deregulation in Ewing sarcoma, and uncover numerous candidate oncogenes which can be exploited for further understanding of the molecular pathogenesis for this disease.