Open AccessHigh-throughput detection and multiplex identification of cell contaminations
Author(s) -
Markus Schmitt,
Michael Pawlita
Publication year - 2009
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gkp581
Subject(s) - multiplex , biology , contamination , oligonucleotide , multiplex polymerase chain reaction , cell culture , high throughput screening , cell , computational biology , microbiology and biotechnology , polymerase chain reaction , chromatography , dna , biochemistry , genetics , gene , chemistry , ecology
Unnoticed cell culture contamination by viruses, Mycoplasma, or other cell lines is not uncommon and a threat to laboratory safety and the quality of scientific results. We developed and validated a novel high-throughput Multiplex cell Contamination Test (McCT), which is currently able to detect 37 contamination markers in a single reaction. The assay is based on multiplex PCR with target-specific primers and subsequent hybridization of amplimers to specific oligonucleotide probes. McCT proved to be highly specific, sensitive and robust, and allows to analyze more than 1000 cell lysates per week. In conclusion, the novel McCT assay is a powerful high-throughput tool in assessing cell line purity.
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