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New genetic tools and strategies are currently under development to facilitate functional genomics analyses. Here, we describe an active member of the  Tc1/mariner  transposon superfamily, named  ZB , which invaded the zebrafish genome very recently.  ZB  exhibits high activity in vertebrate cells, in the range of those of the widely used transposons  piggyBac  ( PB ),  Sleeping Beauty  ( SB ) and  Tol2 .  ZB  has a similar structural organization and target site sequence preference to  SB , but a different integration profile with respect to genome-wide preference among mammalian functional annotation features. Namely,  ZB  displays a preference for integration into transcriptional regulatory regions of genes. Accordingly, we demonstrate the utility of  ZB  for enhancer trapping in zebrafish embryos and in the mouse germline. These results indicate that  ZB  may be a powerful tool for genetic manipulation in vertebrate model species.

A native, highly activeTc1/marinertransposon from zebrafish (ZB) offers an efficient genetic manipulation tool for vertebrates